Mutations in CDRs and FRs before and after the panning selection.

<p>^a Percentage of the designed CDR sequences in total unselected or selected CDR sequences.</p><p>^b Average number of mutations from the closest germline CDR sequence or the reference framework sequence per amino acid position.</p><p>^c<i>P</i> > 0.15 between the values for selected and unselected CDR sequence.</p><p>^d<i>P</i> < 0.05 between the values for selected and unselected FR sequences.</p><p>^e Designed CDR-H3 sequences have no germline ancestors.</p><p>Mutations in CDRs and FRs before and after the panning selection.