E-selectin knockdown in EC-Bcl-2 or FAK/FUT3 knockdown in tumor cells reverses EC-Bcl-2-mediated tumor metastasis.

<p><b>A;</b> E-selectin expression was knocked down in EC-Bcl-2 cells by siRNA. <b>B;</b> FUT3 and FAK expression was knocked down in tumor cells (UM-SCC-74B or CAL27) by siRNA. EC-Bcl-2 transduced with scrambled control siRNA (EC-Bcl-2-SC) or tumor cells transduced with scrambled control siRNA (CAL27-SC) were used as control. <b>C-F;</b> Tumor cells along with EC-Bcl-2 cells were co-injected into the tail veins of SCID mice. <b>C;</b> Representative images of lungs from animals injected with <b>(a)</b> CAL27 and EC-Bcl-2 cells, <b>(b)</b> CAL27-FUT3 knockdown and EC-Bcl-2- E-selectin knockdown cells and <b>(c)</b> CAL27- FAK knockdown and EC-Bcl-2 cells. <b>D;</b> Histological evaluation of lungs from animals injected with <b>(a)</b> CAL27 and EC-Bcl-2 cells, <b>(b)</b> CAL27-FUT3 knockdown and EC-Bcl-2- E-selectin knockdown cells and <b>(c)</b> CAL27- FAK knockdown and EC-Bcl-2 cells. Metastatic nodules in lungs are highlighted by black arrows. The scale bar represents 100 μm. <b>E-F;</b> the number of metastatic nodules in the lungs were counted under microscope. *, represents a significant difference (p<0.05).