Effect of the strength of the acceptor splice site in exon 9 splicing.

<p>The natural 3’ss of the exon 9 in each minigene was substituted with a more functional one by site-directed mutagenesis (c.889-10_889-9delinsTT). The proportion of transcripts including exon 9 was assessed and normalized to the wild type minigene. The stripped and solid bars represent the exon 9 inclusion from the different minigenes with the natural 3’ss and the 3’ss mutated, respectively. All the differences were statistically significant (*** p<0.001).