Results of the splicing minigene analyses.

<p>RT-PCR analysis of the literature-derived E⁺¹ variations. The splicing affecting sequences are underlined. (A) The test set sequences. cDNA bands originating from <i>BTK</i> exon 10 mutated minigene are numbered as follows: 1) cryptic 3′ss utilization 31 nt upstream of the authentic splice site (the aberrant exon starts at c.840-31G), 2) normally spliced RNA. (B) The borderline set sequences.