SomA interacts with PtaB in <i>A</i>. <i>fumigatus</i>.

<p>(A) The abundance of SomA and PtaB was measured by LC/MS and estimated based on MaxQuant’s logarithmized label free quantification (log2 LFQ) intensities. High and intermediate LFQ intensities are shown in red and blue. Absence of peptides and low LFQ intensities are presented in black. (B) Western hybridization of GFP-Trap and RFP-Trap enrichments with α-GFP antibody. The single band in the RFP-Trap indicated by an arrow was identified as SomA-GFP by LC/MS with the given peptides. (C) Reciprocal western to (B) but an α-RFP antibody instead of the α-GFP antibody as a different probe. The double band (arrow) correspond to PtaB and SomA by LC/MS. For all experiments, the strains were grown in MM medium for 24 h at 37°C. Protein extracts were performed with either GFP-Trap or RFP-Trap beads and the eluted proteins were separated by 12% SDS-PAGE.