Deletions suppressing cell lysis in Δ<i>ura4</i> strains grown on YPD medium.

<p> (A) L972 (<i>ura4</i>⁺), UMP31 (Δ<i>ura4</i>), KT35 (<i>ura4</i>-<i>D18</i>), and indicated deletion mutants (Bioneer deletion library ver4.0) were grown for 12 h, then spotted onto YPD medium and incubated for 3 days at 30°C. For the alkaline phosphatase assay, YPD plates were overlaid with a phosphatase assay solution containing 50 mM glycine-NaOH (pH 9.8), 1% agar, and 2.5 mg/ml of BCIP for 30 min. (B) Cells were also observed by microscopy after 3 days of growth on YPD. Five examples of mutants exhibiting different levels of lysis suppression are shown. Bar: 10 μm.