Effect of adenine induced AMPK activation and monocyte adhesion by APRT in HUVECs.

<p>(A) shNT HUVECs or shAPRT HUVECs were treated with various concentration of adenine for 6 h. Cell lysates collected from each condition were used to determine the phosphorylation of AMPK and ACC by western blot using antibodies specific for the phosphorylated protein. (B) shNT HUVECs or shAPRT HUVECs were exposed to 10 μg/L of TNF-α for 6 h in the presence or absence of 600 μM adenine. Top: representative images depicting monocytes to the HUVEC cells as described in “Material and methods”, Scale bar: 200 μM. Bottom: quantification of monocyte adhesion to HUVEC cells. Statistical significance was determined by one-way ANOVA followed by Tukey post hoc tests; all data are plotted as mean ± S.E.M. (n = 5). ***, <i>P</i><0.001; N.S., no significance.