Monocytes adhesion assay.

<p>(a) THP-1 monocytic cells were preincubated with PBS for 0.5 h at 37°C followed by perfusion into a flow chamber. The rolling (attachment) of THP-1 with HUVECs (without TNF-α activation) were recorded and captured. (b, c) THP-1 monocytic cells were preincubated with PBS (b) or IND02 (200 μg/mL) (c) for 0.5 h at 37°C followed by perfusion into flow chamber. The rolling (attachment) of THP-1 with TNF-α activated HUVECs were recorded and captured. (d) Control (without TNF-α activation): 9.86±1.81 cells/mm²; Control (TNF-α activated HUVEC): 378.5±11.5 cells/mm²; IND02 (200 μg/mL): 286.3±17.3 cells/mm². The number of adherent cells was calculated in 10–15 random fields in a single experiment, and the statistical test were calculated based on 3–5 experiments using one-way ANOVA followed by post hoc test. The movie of (a)-(c) were showed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130389#pone.0130389.s002" target="_blank">S1</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130389#pone.0130389.s004" target="_blank">S3</a> Movies. IND02 significantly reduced THP-1 attachment and rolling on TNF-α activated HUVECs. (***: <i>P</i> < 0.001).