NurA/HerA complex activity on shorter linear single-stranded DNA molecules.

<p>(A) 7.5 pmoles of NurA (lanes 2 and 10) or increasing amounts of HerA, from 5 to 12 pmoles (lanes 3–5 and 11–13) have been analyzed separately for their nuclease activity using a linear 17 mer labeled with TAMRA at its 3’- (left) or 5’- (right) ends. The two proteins were then mixed together to analyze the complex activity on these substrates (lanes 6–8 and 14–16). (B) The same experiment was performed using a 21mer single-stranded oligonucleotide labeled at its 3’-end with TAMRA (left) or with Cy5 at its 5’-end (right). 7.5 pmoles of NurA alone (lanes 2 and 10) or increasing amounts of HerA, from 5 to 12 pmoles (lanes 3–5 and 11–13) were analyzed separately. Successively the two proteins were mixed together (lanes 6–8 and 14–16) to analyze the complex nuclease activity. Lanes 1 and 9 are controls in which the only substrate has been run to observe its migration.