NurA D58A mutant activity assays.

<p>(A) Increasing amounts (2.5, 5 and 10 pmoles) of NurA D58A were analyzed for their nuclease activity, on single-stranded DNA, separately (lanes 2–4 and 10–12) or in presence of 12 pmoles of HerA (lanes 5–7 and 13–15) on two different substrates ODN1 and ODN2. Lanes 1 and 9 refer to controls in which the only substrate has been loaded, while lanes 8 and 16 refer to positive controls in which 12 pmoles of NurAwt were incubated in presence of 12 pmoles of HerA. (B) Increasing amounts (2, 6 and 10 pmoles) of NurA D58A were analyzed for their nuclease activity, on linear double-stranded DNA, in presence of 12 pmoles of HerA (lanes 4–6); lane 1 refers to control in which the only substrate has been loaded, lane 2 refers to NurA D58A alone (10 pmoles) and lane 3 refers to HerA alone (12 pmoles).