<p>Fluorescence microscopy of CYC3-GFP at different time points: 5, 7, 10, 14 and 21 dpi during development in the mosquito. Scale bar = 20 μm. Representative percentage of oocysts that either: do not express GFP (black number), have a low expression of GFP (red number) or have a high expression of GFP (green number).</p
<p>Two independently prepared mutants were used for assessing the phenotype in each gene. The number...
<p>(A) Ookinete numbers were determined by light microscopy analysis of midguts dissected at 24 h.p....
<p>A: Midguts from 10 infected or uninfected mosquitoes were dissected on days 7–9 post feeding. Lys...
<p>(A) Average number of nuclei per schizont measured using Giemsa stained slides at 100x magnificat...
<p>(A) For blood stage images, blood of infected mice was diluted and stained with the blue nuclear ...
<p><b>A.</b> Photographs of representative mosquito midguts on days 14 and 21 post infection. Contro...
<p><b>A.</b> Oocyst numbers. On day 14 post-infection, midguts from 20–30 mosquitoes were scored for...
<p>A) Transgenic lines expressing the GFP reporter under the control of the upstream regulatory regi...
<p>(A) Mercurochrome staining of oocysts in the midgut preparations. Arrows indicate oocysts and the...
<p>(A) Transcription of <i>cyc1</i>, <i>cyc3</i> and <i>cyc4</i> as analysed by qRT-PCR, normalised ...
<p>A. Fluorescence microscopy of different blood stages. R: rings; T: trophozoites; ES: early schizo...
<p>A) Exflagellation in wild type (WT-GFP) and transgenic Pb<i><sup>pfpkg</sup></i> parasites. The m...
(a) Exflagellation of actIIH73Q was comparable to WT. Exflagellation tests were carried out three ti...
<p>(A) Oocyst formation of NcPI-S expressing clones (<i>NcPIS_3</i> and <i>NcPIS_1</i>) compared to ...
(A) Tissue-specific and developmental expression pattern of EOF1 during the first gonotrophic cycle ...
<p>Two independently prepared mutants were used for assessing the phenotype in each gene. The number...
<p>(A) Ookinete numbers were determined by light microscopy analysis of midguts dissected at 24 h.p....
<p>A: Midguts from 10 infected or uninfected mosquitoes were dissected on days 7–9 post feeding. Lys...
<p>(A) Average number of nuclei per schizont measured using Giemsa stained slides at 100x magnificat...
<p>(A) For blood stage images, blood of infected mice was diluted and stained with the blue nuclear ...
<p><b>A.</b> Photographs of representative mosquito midguts on days 14 and 21 post infection. Contro...
<p><b>A.</b> Oocyst numbers. On day 14 post-infection, midguts from 20–30 mosquitoes were scored for...
<p>A) Transgenic lines expressing the GFP reporter under the control of the upstream regulatory regi...
<p>(A) Mercurochrome staining of oocysts in the midgut preparations. Arrows indicate oocysts and the...
<p>(A) Transcription of <i>cyc1</i>, <i>cyc3</i> and <i>cyc4</i> as analysed by qRT-PCR, normalised ...
<p>A. Fluorescence microscopy of different blood stages. R: rings; T: trophozoites; ES: early schizo...
<p>A) Exflagellation in wild type (WT-GFP) and transgenic Pb<i><sup>pfpkg</sup></i> parasites. The m...
(a) Exflagellation of actIIH73Q was comparable to WT. Exflagellation tests were carried out three ti...
<p>(A) Oocyst formation of NcPI-S expressing clones (<i>NcPIS_3</i> and <i>NcPIS_1</i>) compared to ...
(A) Tissue-specific and developmental expression pattern of EOF1 during the first gonotrophic cycle ...
<p>Two independently prepared mutants were used for assessing the phenotype in each gene. The number...
<p>(A) Ookinete numbers were determined by light microscopy analysis of midguts dissected at 24 h.p....
<p>A: Midguts from 10 infected or uninfected mosquitoes were dissected on days 7–9 post feeding. Lys...