CYC3-GFP protein expression throughout most stages of the life cycle.

<p>(A) Transcription of <i>cyc1</i>, <i>cyc3</i> and <i>cyc4</i> as analysed by qRT-PCR, normalised against two endogenous control genes, <i>arginine-tRNA synthetase</i> and <i>hsp70</i>. Each bar is the mean of three biological replicates ± SEM. All asexual blood stages: AS; schizonts: Sch; non-activated gametocytes: NAG; activated gametocytes: AG; ookinete: Ook; 14 dpi oocysts/sporozoites: Spor. (B) Expression of CYC3-GFP in trophozoites, schizonts, gametocytes, zygotes, ookinetes, oocysts and sporozoites. 13.1, a cy3-conjugated antibody which recognises P28 on the surface of activated females, zygotes, and ookinetes was used with the sexual stages. Scale bar = 5 μm. (C) Deconvolved 2D projections of live trophozoite, gametocyte, and ookinete expressing CYC3-GFP (green), co-stained with Hoechst 33342 (blue) and cy3-conjugated anti-P28 antibody, 13.1 as a marker for the ookinete surface (red). Scale bar = 5 μm. Line profiles (red) in the black and white images indicate pixel intensity for that channel.