Morphologic and immunophenotypic characteristics of suspicious circulating elements in healthy donors.

<p>Representative image of a hematoxylin stained nucleus isolated by depletion procedure (A). A DAPI stained nucleus isolated by depletion procedure and small contaminant white blood cells (B). Confocal image of a propidium iodide (PI) stained nucleus obtained by depletion procedure (C) and a hematoxylin and eosin stained nucleus isolated by the ScreenCell device (D). E—G: cytospin preparations obtained after depletion procedure and tested by immunocytochemistry showing a vimentin positive cell associated with a negative leukocyte (E), a CD31 immunostained cell with a negative leukocyte (F) and two CD68 positive and one CD68 negative cells (G). Scale bars: A, E, F, G = 10 μm; B, D = 20 μm; C = 5 μm.