Representative, confocal images of a cytospin of an enriched peripheral blood sample from a 27 year old, HPV positive, SCCHN patient.

<p>Sample taken in the OR, 214 cytokeratin positive cells per ml of blood were detected, and she had a reoccurrence 2 months after surgery. For this specific four color staining, the cell structure/protein targeted is listed in each image. The cells highlighted with a red arrow are positive for all four markers, yellow are negative for CK, weak or negative for vimentin and EGFR, and white is negative for all but nuclei. Green arrows highlight cells that have a granulocytic like nuclei.