Phylogenetic analysis using the MrBayes algorithm.

<p>The original tree includes many members that cannot easily be displayed on one page. The individual parts of the tree have therefore been enlarged and presented separately (Figs <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143091#pone.0143091.g007" target="_blank">7</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143091#pone.0143091.g010" target="_blank">10</a>) for the chymase locus-related genes, the fish chymase-related genes, the met-ase locus genes and the granzyme A/K locus genes, respectively. The chymase locus-related fish genes that are found within the met-ase locus (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143091#pone.0143091.g010" target="_blank">Fig 10</a>) are marked by green instead of blue. Human and mouse coagulation factor X and complement factor B have been used as outgroups to get a more distinct topology of the tree. The three catfish serine proteases that are under more detailed biochemical analyses are marked by red stars in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143091#pone.0143091.g009" target="_blank">Fig 9</a>. They represent three of the four major sub-branches of fish chymase-locus related serine protease genes. The posterior probability values are shown at each branch point in the tree.