Schematic Representation of the A. CHIKV and B. DENV genomes, respectively, with regions targeted by the Dual Targeting Introns.

<p><b>A</b>. CHIKV genomic RNA diagram with the indicated conserved region targeted for viral suppression. The CHIKV conserved domain, labeled as the CHIKV Conserved Region, was determined through a Clustal X alignment of GenBank obtained CHIKV sequences (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0139899#sec011" target="_blank">Materials and Methods</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0139899#pone.0139899.s005" target="_blank">S5 Fig</a>). <b>B</b>. The most invariant segments of the DENV RNA are known as the 5’ and 3’ cyclization sequences (labeled as the Group I Intron target site). These sequences are so named because they are complementary to each other and are thought to be involved in the formation of a panhandle structure during genome replication.