siRNA targets in the DENV NTRs.

<p>A ClustalW2 multiple alignment of 5′ (A) and 3′ (B) NTR sequences of the prototypic representatives of DENV-1, -2, -3 and -4 (described in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001735#s2" target="_blank">Methods</a>) showing the sites conserved in two or more serotypes targeted for RNAi in this study. NTR sequences that were utilized to design the sense strand of the sh constructs are shown in red fonts. The names of the sh constructs are shown in italics above the sequences in red fonts. The DENV-4 5′NTR seed sequence identical to the sh-5b target site is underlined.