Expression of other heterologous recombinant proteins.

<p>BL21(DE3) cells were transformed with pSA-Nef/Vif-Bla or pSA-Nef/Vif-FabV plasmids and cultured in LB broth supplemented with either 269 μM (100 μg mL^-1) Ampicillin or 1 μM Triclosan respectively. Protein expression was induced with 50 μM IPTG when cultures reached to 0.5 OD600. Induced and uninduced cultures were grown at 30°C for 6 hours and then analyzed by SDS-PAGE, western blot, and densitometry for the expression of recombinant Nef (A) and Vif (B) proteins. Error bars show standard deviations calculated from at-least three independent experiments each performed in duplicate.