Comparison of plasmid stability/retention between Bla and FabV vectors.

<p>BL21(DE3) cells were transformed with Bla or FabV vectors and uninduced and IPTG -induced cultures were grown at 37°C/4h, 30°C/6h, and 22°C/12 h in presence of Ampicillin (for bacteria containing Bla vectors) or Triclosan (for bacteria containing FabV vectors). Cultures were diluted and plated on non-selective LB agar plates. After 18 h incubation at 30°C, 100 colonies were replica patched on non-selective and selective (with Ampicillin or Triclosan) LB agar plates, incubated at 30°C for 18 h, and counted. (A) cultures expressing P24 protein; (B) cultures expressing Nef protein; (C) cultures expressing Vif protein. Error bars show standard deviations calculated from three independent experiments each performed in duplicate.