Mdm2 interacts with TFII-I.

<p>(A) HEK293T cells were transfected with a plasmid coding for human Mdm2. Endogenous TFII-I was co-immunoprecipitated from cell lysates using anti-Mdm2 antibody. (B) U2OS cells were transfected with a plasmid coding for human Mdm2. The subcellular localization of endogenous TFII-I and of the ectopically expressed Mdm2 was determined by immunofluorescence. DAPI was used to label cell nuclei. (C) Endogenous TFII-I and Mdm2 were co-immunoprecipitated from H1299 cell extracts using anti-TFII-I and anti-Mdm2 mouse monoclonal antibodies. Anti-tubulin α antibody served as a negative control. (D) Plasmids coding for GST and GST-tagged TFII-I mutant lacking the nuclear localization signal (ΔNLS) were transfected into U2OS cells either alone or together with Mdm2. Subcellular localization of the GST protein tag, GST-TFII-IΔNLS, and Mdm2 was analyzed using immunofluorescence. DAPI staining was used to label cell nuclei.