Unaltered prion pathogenesis in HFD-fed <i>tg</i>a<i>20</i> mice.

<p>(<b>A</b>) Survival curve of HFD-fed and ND-fed <i>tg</i>a<i>20</i> mice intracerebrally inoculated with RML6. There was no significant difference in survival between the two groups (n = 6 per group; n.s.: <i>p</i>>0.05). (<b>B</b>) Left: Western blot for proteinase K resistant PrP^Sc in terminally sick mouse brains. Right: densitometric quantification of the Western blot revealed no significant difference between HFD-fed and ND-fed <i>tg</i>a<i>20</i> mouse brains (n = 3, n.s <i>p</i>>0.05). (<b>C</b>) Representative histology of terminally sick HFD-fed and ND-fed <i>tg</i>a<i>20</i> mouse brains stained for H&E and SAF84. There was no obvious difference between the two groups in lesion pattern, vacuolation, and PrP^Sc deposition. Scale bar = 200μm.