EP2 enhanced PAP248-286 fibril formation and promoted the attachment of HIV-1 virions to target cells.

<p>(A) ProteoStat-stained amyloid fibrils at different time points following agitation (100 μg/ml, red) were mixed with eGFP-labeled HIV-1 virions (green, R5 strains) for 3 h, and the samples were imaged using fluorescence confocal microscopy. The scale bar is 5 μm. (B) ProteoStat-stained amyloid fibrils at different time points following agitation (200 μg/ml, red) were added to eGFP-labeled HIV-1 virions (green, R5 strains), and the mixtures were added to GHOST (3) Hi-5 cells (blue). The effects of fibril presence on the binding of HIV-1 virions to target cells were assessed using fluorescence confocal microscopy. The scale bar is 10 μm.