Mutation of <i>VdNUC-2</i> resulting in altered colony morphology in Pi-deficient Czapek–Dox media.

<p>A, Colony morphologies of wild-type <i>V</i>. <i>dahliae</i> (V07DF2), T-DNA insertion mutant (6C4), two targeted deletion mutants (<i>VdNUC-2Δ5</i> and <i>VdNUC-2Δ14</i>), and two complementation strains (VdNUC-2C6 and VdNUC-2C7) after 14 days of incubation on normal Pi (Con _Pi = 5.7 mM), low Pi (Con _Pi = 57 μM), and Pi-free Czapek–Dox (Con _Pi = 0 mM) media. B, C, and D, Colony diameters measured at indicated days after inoculation on culture media containing different Pi contents. Error bars represent standard deviation (<i>n</i> = 4). E, Reduced aerial hyphae growth in <i>VdNUC-2</i> mutants under low Pi culture conditions. The pictures were obtained after 14 days of incubation. Bar = 1 cm.