Effect of curcumin on BK protein abundance and BK mRNA in A7r5 cells.

<p>(A) Western blot analysis showed that treatment with curcumin at 0, 2.5, 5, 10, and 20 μM for 24h up-regulated the BK protein expression in A7r5 cells. * <i>p</i> <0.05 (n = 3). Graphic representative of densitometric data of the above total BK protein level are shown in (B). (C) Real-time RT-PCR for the measurement of BK mRNA levels with 10 μM curcumin treatment; the BK mRNA levels were normalized with GAPDH. The predicted amplicon size for BK and GAPDH is 103 bp and 271 bp, respectively. n.s., not significant (n = 3).