Curcumin inhibits γ-secretase complex proteins.

<p>(A) real-time reverse transcription-PCR analysis of total RNA from TE-7 cells following 30 µM of curcumin treatment for 24 h showed reduction in the expression of Presenilin 1 and Nicastrin mRNA (*p<0.05). (B) lysates from curcumin treatment caused significant reduction in the expression of γ-secretase complex proteins Presenilin 1 and 2, Nicastrin, APH1 and Pen2 protein levels in TE-7 cells. (C) TE-7 cells treated with 30 µM of curcumin for 24 h were subjected to immuno-fluorescent staining using anti-Presenilin 1 and anti-Nicastrin antibodies. Curcumin treatment resulted in lower levels of Presenilin 1 and Nicastrin expression in TE-7cells.