Viability assay of non-treated and chemoresistant ovarian cancer cell lines challenged with carboplatin, NEDDylation inhibitor MLN4924 or both.

<p>The ES-2 ovarian cancer cell line was pre-treated with 50 μM carboplatin and the surviving cells were selected as chemoresistant to carboplatin. The chemoresistant and non-treated cells were challenged with 500 μM carboplatin, 10 nM MLN4924 or a combination of both for 24 hours. The results are presented as mean and SD, analysed by one-way ANOVA–multiple comparisons, with statistical significant p = 0.0001, using GraphPad software. The capped lines represent the group of samples that were not statistically different between each other, and the asterisks represent a statistically significant difference between the groups that were compared.