De-differentiation of serum-differentiated GG16 cells reduces their invasive potential.

<p><b>a.</b> Heatmap based on fold change of the indicated stem cell and differentiation markers generated by qRT-PCR analyses showing partial gain of stem cell and loss of differentiation markers following dedifferentiation of GG16 cells. <b>b.</b> Representative transwell membranes (magnification x5) showing reduced numbers of invading cells after dedifferentiation of GG16 cells (secondary Nsp) that is almost equal to that of primary neurosphere cells; quantification in <b>c.</b> showing mean ± SEM of 3 independent experiments (*<i>P<0</i>.<i>05)</i>. <b>d.</b> Western blot showing the absence of MMP9 expression in primary and secondary GG16 neurosphere cells in comparison to the differentiated GG16 cells.