qPCR validation of RNA-seq result.

<p>Quantitative PCR for (A) <i>gli1</i>, (B) <i>ptch1</i>, and (C) <i>hsd11b1</i> was performed on Hh ligand treated and Hedgehog ligand and SFE co-treated TRAMPC2 cells. Transcripts concentrations were normalized to control. * indicates p<0.05. In the lower figure, transcripts concentrations of (A) <i>gli1</i>, (B) <i>ptch1</i>, and (C) <i>hsd11b1</i> are represented by quantified sequencing reads, in the form of counts-per-million-reads.