TMF^-/- round spermatids lack the CB compartment.

<p>Testicular sections from wt (A and C) or TMF^-/- (B and D) mice were subjected to TEM analysis. (A) Stage 4 round spermatid of wt mice. The Golgi apparatus is marked by an arrow. The CB is marked by an arrow head. (B) Stage 4 TMF^-/- round spermatid. The Golgi is marked by an arrow. No CB is observed in this spermatid. (C) Early stage 1 spermatid of wt mice. The Golgi is marked by an arrow. The CB is marked by an arrow head. (D) TMF^-/- spermatid of an early stage 1. The Golgi is marked by an arrow. No CB is observed in this spermatid. Bars represent 2μm. (E and G) Testicular round spermatids of wt mice were subjected to KIF17B immunofluorescence staining using specific antibodies (green, aggregations of KIF17B are marked by arrows). (F and H) TMF^-/- testicular round spermatids were subjected to KIF17B immunofluorescence staining using specific antibodies (green). Nuclei were visualized with Hoechst. Bars represent 10μm.