Assessment of plaque burden and plaque morphology.

<p>Frozen sections through the aortic sinus of both control mice and Dagla-deficient mice were stained using oil red O to visualise atherosclerotic plaques (A, B). Cumulative plaque area was divided by the size of the vessel wall to obtain relative plaque burden (C). Neutrophil granulocytes were identified by staining Ly6G (D, E). Ly6G positive areas were normalised to the size of the vessel wall (F). Macrophages were identified by staining CD68 (G, H). CD68 positive areas were normalised to the size of the vessel wall (I). Data are presented as mean ± standard error of the mean; n = 9–13; *, p < 0.05. Scale bar, 200 μm. CD, cluster of differentiation; cre, cyclisation recombination; Dagla, diacylglycerol lipase α; fl, flanked by loxP; Ly6G, lymphocyte antigen 6G; LysM, lysozyme 2 gene.