Local difference of H3K27me3 marks in mutants of PcG components.

<p>(A) ChIP-seq density heatmaps in Col-0 and PcG mutants, ranked by H3K27me3 read intensity within ±500 bp of peak summits in Col-0. The Pearson correlation coefficient between H3K27me3 intensity in Col-0 and FIE binding within ±50 bp of peak summits is 0.52. ChIP-seq data of H3K27me3 in panel II and FIE binding in panel VII were published previously [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005771#pgen.1005771.ref040" target="_blank">40</a>,<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005771#pgen.1005771.ref041" target="_blank">41</a>]. To compare H3K27me3 level with the bindings of LHP1 and EMF1 based on published ChIP-chip data [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005771#pgen.1005771.ref015" target="_blank">15</a>,<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005771#pgen.1005771.ref032" target="_blank">32</a>], the fraction of EMF1 peaks (blue) or LHP1 peaks (red) Col-0 overlapped with Col-0 H3K27me3 peaks were plotted. (B) RNA-seq intensity heatmap for the targets of H3K27me3 peaks in Col-0 with the same order as in (A). The expression intensity is measured by Reads Per Kilobase per Million of mapped reads (RPKM). (C) (C) IGV screen shots showing the distribution of H3K27me3 mark intensity in Col-0 and different mutants of PcG components. Negative control is Col-0 sample immunoprecipitated with beads but without antibody. Regions showing quantitative reduction of H3K27me3 marks are highlighted by blue box.