Double immunofluorescence showing expression of IFNγ (green) and SMemb (red) in thin sections of uninjured coronaries, post PTCA-coronary arteries, uninjured SEV and SEV-graft.

<p>Less immunopositivity to IFNγ (green) and SMemb (red) are noted in uninjured coronary artery at low (20x) (A) and high (40x) magnification (B, C, D). Strong expression of IFNγ (green) and SMemb (red) are found in the hyperplastic region post PTCA- coronary arteries (arrows) at low (20x) (E) and high (40x) magnification (F, G, H). Similarly, less immunostaining of IFNγ (green) and SMemb (red) expression is noted in the uninjured SEV at low (20x) (I) and high (40x) magnification (J, K, L) compared to SEV post CABG, where many cells showed immunopositivity to IFNγ (green) and SMemb (red) at low (20x) (M) and high (40x) (N,O,P) magnification. This is a representative of tissue histology in 2–5 individual swine in each experimental group. MFI measured in arbitrary units (AU) in post-PTCA coronary arteries (Q) and SEV-grafts (R) compared to their uninjured counterparts. Graphs showing number of IFNγ - positive (+) cells in the neointima of the post-PTCA restenotic coronary artery (S) and SEV-grafts (T) compared to their uninjured counterparts. Many cells in the neointima of the injured vessels were immunopositive for both, IFNγ and SMemb, especially around the pseudo lumen in SEV-graft. Data are shown as mean ± SEM of values of at least three measurements in each group in 2–5 individual swine in each experimental group. *P < 0.05. L: lumen, M: medial layer, NI: neointima.