Double immunofluorescence showing expression of pStat-3 (green) and PLK1 (red) in thin sections of uninjured coronaries, post PTCA-coronary arteries, uninjured SEV and SEV-graft in swine.

<p>Less immunopositivity to pStat-3 (green) and PLK1 (red) are noted in uninjured coronary artery at low (20x) (A) and high (40x) magnification (B, C, D). Strong expression of pStat-3 (green) and PLK1 (red) are found in the hyperplastic region post PTCA- coronary arteries (arrows) at low (20x) (E) and high (40x) magnification (F, G, H). Similarly, less immunostaining of pStat-3 (green) and PLK1 (red) expression is noted in the uninjured SEV at low (20x) (I) and high (40x) magnification (J, K, L) compared to SEV post CABG, where many cells showed immunopositivity to pStat-3 (green) and PLK1 (red) at low (20x) (M) and high (40x) (N,O,P) magnification. This is a representative of tissue histology in 2–5 individual swine in each experimental group. MFI measured in arbitrary units (AU) in post-PTCA coronary arteries (Q) and SEV-grafts (R) compared to their uninjured counterparts. Graphs showing number of pStat-3—positive (+) cells in the neointima of the post-PTCA restenotic coronary artery (S) and SEV-grafts (T) compared to their uninjured counterparts. Increased immunopositivity towards pStat-3 and PLK1 are found in the neointimal region of the injured vessels compared to the uninjured vessels. This is a representative of tissue histology in 2–5 individual swine in each experimental group. L: lumen, M: medial layer, NI: neointima.