Construct schematic. Schematic representation of the Zoo-2 vector [30], linearized at the 3′ end of the PiggyBac transposon. The inserted sequences enhance the efficiency of intron splicing. The two functional segments include the marker (Act5C promoter, DsRed-2, Drosomycin 3′-UTR) and a tra-2 RNAi cassette (tetO7-CMV minimal promoter, RRM inverted repeats joined by the tra-2 intron and rabbit β-globulin 3′-UTR). (TIF 1813 kb
Figure S1. (PDF) Sequence of the Nix locus showing the positions of the exons (green box), intron an...
(A) Schematics of putative exon-intron structures of the sex determination genes in T. dichotomus. O...
Expression profile of KLC2 (AAEL011410) based on RNA sequencing data adopted from (a) Biedler et al....
Schematic representation of the homologous TRA-2 proteins. (A) Protein encoded by the AAEL009224 gen...
The expression of tra-2 in wild-type males and tra-2 RNAi transgenic males. Quantitative real-time P...
Figure S1. Alignment of the 207 kb BAC region to the corresponding region in the AaegL5 male referen...
The accession numbers of the RRM sequences: [GenBank: KJ147314, KJ147315, KJ147316, KJ147317, KJ1473...
Genes with significant sex-association values common to both populations. Vectorbase number (VBN), t...
Table S1. Types and abundance of repeats in the 207kb assembled M locus region and 99 kb Nix intron,...
Luciferase expression in seven independent transgenic lines. Firefly luciferase assays showed that e...
Alignment of RNAseq data [29] to the genomic region surrounding the insertion site. The grey bars in...
Sexual dimorphism in expression of genes significantly different in RNAseq experiments. (A) Number o...
Aedes aegypti is one of the most important mosquito vectors of human disease, transmitting dengue, C...
Figure S2. Quantification of CG42724 steady-state mRNA levels by RT-QMPSF. (A) Schematic representat...
Figure S1. The expression profile of RNAi across tissue. The normalized expression plotted across ti...
Figure S1. (PDF) Sequence of the Nix locus showing the positions of the exons (green box), intron an...
(A) Schematics of putative exon-intron structures of the sex determination genes in T. dichotomus. O...
Expression profile of KLC2 (AAEL011410) based on RNA sequencing data adopted from (a) Biedler et al....
Schematic representation of the homologous TRA-2 proteins. (A) Protein encoded by the AAEL009224 gen...
The expression of tra-2 in wild-type males and tra-2 RNAi transgenic males. Quantitative real-time P...
Figure S1. Alignment of the 207 kb BAC region to the corresponding region in the AaegL5 male referen...
The accession numbers of the RRM sequences: [GenBank: KJ147314, KJ147315, KJ147316, KJ147317, KJ1473...
Genes with significant sex-association values common to both populations. Vectorbase number (VBN), t...
Table S1. Types and abundance of repeats in the 207kb assembled M locus region and 99 kb Nix intron,...
Luciferase expression in seven independent transgenic lines. Firefly luciferase assays showed that e...
Alignment of RNAseq data [29] to the genomic region surrounding the insertion site. The grey bars in...
Sexual dimorphism in expression of genes significantly different in RNAseq experiments. (A) Number o...
Aedes aegypti is one of the most important mosquito vectors of human disease, transmitting dengue, C...
Figure S2. Quantification of CG42724 steady-state mRNA levels by RT-QMPSF. (A) Schematic representat...
Figure S1. The expression profile of RNAi across tissue. The normalized expression plotted across ti...
Figure S1. (PDF) Sequence of the Nix locus showing the positions of the exons (green box), intron an...
(A) Schematics of putative exon-intron structures of the sex determination genes in T. dichotomus. O...
Expression profile of KLC2 (AAEL011410) based on RNA sequencing data adopted from (a) Biedler et al....