Additional file 3: Figure S2. of Mechanisms of sex determination and transmission ratio distortion in Aedes aegypti

The expression of tra-2 in wild-type males and tra-2 RNAi transgenic males. Quantitative real-time PCR was also used to detect the level of tra-2 mRNA remaining in homozygous male pupae of line 6 (red) and wild-type Rock male pupae (blue). The tra-2 expression level was reduced by an average of 48.7 % in line 6 relative to wild-type insects in three replicates (Standard Deviation = 4.98) [30]. (TIF 8549 kb