Periostin overexpressiong compromises migration properties of MC3T3-E1 cells.

<p>(a) MC3T3-E1 periostin-overexpressing (P) and control cells (C) were allowed to migrate simultaneously over a 500 μm gap in standard culture dishes commonly employed for wound healing assays (untreated) or wells coated with type-I collagen or fibronectin. Pictures at starting (t = 0 h), 4 h, 8 h, 12 h, and 16h time points are included. Starting point is indicated with a straight blue line and final points with a dotted line: yellow for MC3T3-E1 control cells and red for clone P3 cells. (b) Graphical representation of migration rate measured at 8 h from three independent experiments. Student t test (<i>p < 0</i>.<i>05</i>, *; <i>p < 0</i>.<i>01</i>, **; <i>p < 0</i>.<i>005</i>, ***).