Periostin stimulates chemotaxis migration, adhesion, and proliferation activity of hASCs <i>in vitro</i>.

<p>(A) Dose-dependent effect of periostin on hASC migration. hASCs were loaded in the upper chamber of a chemotaxis chamber apparatus and recombinant periostin with the indicated concentration was placed in the lower chamber, followed by measurement of the number of migrated cells after 12 h incubation. (B) Checkerboard analysis of the periostin-induced migration of hASCs was measured using a chemotaxis chamber. Periostin was placed in either the bottom, top, or both chambers of the chemotaxis system and hASCs were loaded into the upper chamber. The number of migrated cells was quantified after incubation of the cells for 12 h. Data represent mean ± S.D.; *, p < 0.05. (C) 96-well plates were coated with indicated concentration of periostin and adhesion of hASCs onto the plates was determined. (D) Dose dependence of periostin-stimulated proliferation. hASCs were treated with the increasing concentrations of periostin for 3 days and proliferation of cells was determined by MTT assay. Data represent mean ± S.D. *, p < 0.05 vs control.