S316 may function as a potential phosphorylation site regulating OSM-3 localization and function.

<p>(A) Kymograph data for IFT velocity in middle and distal segments of worms expressing DYF-11::GFP, OSM-3::GFP and OSM-3(S316F)::GFP. N.S. = not significant, * = p<0.05 (B) Analysis of dye-filling in the indicated strains to determine the function of the potential phospho-null OSM-3(S316F)::GFP and phospho-mimetic OSM-3(S316D)::GFP forms of OSM-3. SMC, single mutant control. DMC, double mutant control. N = 3 trials with at least 100 animals per trial. * = p<0.05 as compared to DMC, # = p<0.05 as compared to SMC <i>osm-5(mn357)</i>. (C) Analysis of the osmotic (Osm) avoidance behavior in the indicated strains to determine the functional consequence of the phospho-null OSM-3(S316F)::GFP and phospho-mimetic OSM-3(S316D)::GFP mutations. DMC, double mutant control. n = 3 trials with at least 50 animals tested per trial. * = p<0.05 as compared to <i>nphp4(tm925)</i>, # = p<0.05 as compared to DMC. (D) Stringent analysis (15 minute) of the Dyf phenotypes in the indicated strains to analyze possible dominant effects of the putative phospho-null OSM-3(S316F)::GFP. * = p<0.05 as compared to <i>nphp4(tm925)</i>. (E) Analysis of phasmid cilia length in wild type, <i>nphp-49tm925)</i>, and <i>nphp-4(tm925);osm-3(yhw66)</i> mutants expressing OSM-3::GFP, OSM-3(S316F)::GFP, or OSM-3(S316D)::GFP transgenes. * = p<0.05 as compared within genotype, # = p<0.05 as compared to <i>nphp-4(tm925)</i> expressing same transgene