Kinetics of Rh1 transport in 546P mutant photoreceptors.

<p>546P mutant mosaic eye immunostained by the following antibodies. RFP (red) indicates wild type cells. Asterisks show 546P mutant photoreceptors. (A) Immunostaining before BLICS. Anti-Rh1 (green) and anti-NinaA antibodies (blue, endoplasmic reticulum marker). (B) Immunostaining 40 min after BLICS. Anti-Rh1 (green) and anti-GM130 (blue, Golgi marker) antibodies. (C) Immunostaining 180 min after BLICS. Anti-Rh1 (green) and anti-Rab7 (blue, late endosome marker) antibodies. (D, E) Immunostaining 30 min (D) and 60 min (E) after BLICS. Anti-Rh1 (green) and anti-GM130 (blue, Golgi marker) antibodies. (F) Immunostaining of a 546P mutant ommatidium 90 min after BLICS. Anti-Rh1 (green), Rab7 (red, late endosome marker) and anti-p120 (blue, Golgi marker) antibodies. (G) Projection image from 20 slices at 0.5-μm intervals of 546P mutant ommatidium 90 min after BLICS. Anti-Rh1 (green), Rab7 (red) and anti-p120 (blue) antibodies. A schematic view of Rh1, Rab7, and p120 is shown under the projection image. (H) Pearson’s correlation coefficients of the co-localization between Rh1 and Rab7, and Rh1 and MyoV as a control. MyoV predominantly localizes on the pigment granules in the photoreceptors. (I) Immunostaining of 546P mutant mosaic eye expressing GFP::MyoV C-terminal dominant-negative protein by Rh1Gal4 driver. Asterisks show 546P mutant photoreceptors. Scale bars: 5 μm (A–G, I). Numbers of the samples observed were shown in the top-left corner of the composite images.