YFP::Rab6wt expression rescues the 546P mutant phenotype.

<p>(A–G) 546P mutant mosaic eyes expressing YFP::Rab6wt driven by heat shock-Gal4 were immunostained with the indicated antibodies. RFP (red) indicates wild type cells. (A) Schematic of the heat shock procedure (arrow) and YFP::Rab6wt expression (pink bar). (B, D, F) Immunostaining of 546P mutant mosaic eyes expressing YFP::Rab6wt driven by heat shock-Gal4. YFP::Rab6wt and Rh1 are shown in green and blue, respectively. Significant autofluorescence was also observed in pigment granules in the green channel. (C, E, G) Immunostaining of a 546P mutant mosaic eye expressing YFP::Rab6wt driven by Rh1-Gal4. YFP::Rab6wt with auto-fluorescence of pigment granules and Eys are shown in green and blue respectively. Significant autofluorescence was also observed in pigment granules in the green channel. (H–J) 546P mutant mosaic eyes expressing YFP::Rab6wt driven by Rh1-Gal4 were immunostained with the indicated antibodies. RFP (red) indicates wild type cells. (H) Schematic view of YFP::Rab6wt expression (pink bar). (I) Immunostaining of 546P mutant mosaic eyes expressing YFP::Rab6wt driven by Rh1-Gal4. YFP::Rab6wt with autofluorescence of pigment granules and Rh1 are shown in green and blue, respectively. (J) Immunostaining of 546P mutant mosaic eyes expressing YFP::Rab6wt driven by Rh1-Gal4. YFP::Rab6wt with autofluorescence of pigment granules and Eys are shown in green and blue respectively. Scale bars: 5 μm (A–J). Numbers of the samples observed were shown in the top-left corner of the composite images.