Transcriptional response following <i>L</i>. <i>monocytogenes</i> infection of WT mice in blood and spleen.

<p><b>(A, B)</b> Heatmaps of the significantly regulated blood and spleen transcripts at day 3 following intravenous injection of C57BL/6 WT mice with 5 × 10³ of <i>L</i>. <i>monocytogenes</i> (<i>p</i> < 0.01 after unpaired <i>t</i>-test with Benjamini–Hochberg multiple testing correction on transcripts passing quality control filtering, <i>n</i> = 10 mice/group). The top five QIAGEN Ingenuity® Pathway Analysis (IPA®) canonical pathways by significance (Fisher’s exact test) for upregulated (red bars) or downregulated (blue bars) genes are listed below the heatmaps. <b>(C)</b> qRT-PCR data on selected transcripts normalized relative to <i>Hprt1</i> expression levels (mean with SD, <i>n</i> = 5 mice/group). Samples are from the same experiment as the microarray data. <b>(D)</b> CD3⁺Thy1⁺ cells in blood and spleen as a percentage of total live cells. Pooled results are from triplicates of 3 independent experiments (mean with SEM, <i>n</i> = 3 mice/group/experiment).