Effect of BPA on 3T3-L1 protein abundance of master differentiation genes.

<p>Protein levels of PPARγ <b>(A)</b>, FABP4/AP2 <b>(B)</b> and GLUT-4 <b>(C)</b> were assayed during adipogenesis at days 4 and 8 by western blot analysis, expressed as Arbitrary Unit (AU). Bars represent the mean ± SD of four independent experiments and blot is representative of four different experiments. Asterisks indicate statistically significant differences (*p<0.05) between days 4 and 8 for PPARγ <b>(A)</b> and day 8 for FABP4/AP2 <b>(B)</b>, without and with BPA incubation, both compared to untreated day 4. Hash (#p<0.05) expresses statistically significant differences between days 4 and 8 for PPARγ <b>(A)</b> and day 8 for FABP4/AP2 <b>(B)</b>, upon BPA incubation compared to controls. No significant differences in GLUT-4 protein expression <b>(C)</b>.