Effect of BPA on 3T3-L1 proliferation and mRNA gene expression.

<p><b>(A)</b> 3T3-L1 pre-adipocyte were counted and expressed as cells/ml, at days 15, 16, 17 and 18, after 2 weeks of incubation with (BPA) or without (CTR) BPA 1nM, before adipogenesis started. PPARγ <b>(B)</b>, FABP4/AP2 <b>(C)</b> and cEBPα <b>(D)</b> mRNA levels were assayed during adipogenesis at days 0, 4 and 8, by Real-time RT-PCR analysis, expressed as Relative Expression Unit (REU). Bars represent the mean ± SD of four independent experiments. Asterisks indicate statistically significant differences (*p<0.05; **p<0.01; ***p<0.001) at days 4 and 8 compared to untreated day 0 for PPARγ <b>(B)</b>, at day 8 compared to untreated day 4 for FABP4/AP2 <b>(C)</b>, and at day 0 and day 4 compared to untreated day 0 for cEBPα <b>(D)</b>, without or with BPA incubation. Hashes (# p< 0.05; ### p<0.001) express statistically significant differences between day 8 with or without BPA incubation <b>(B and C)</b> and between day 0 and day 4 with or without BPA incubation <b>(D)</b>.