Overlapping PCR result demonstrating the amplification of the full-length vector with the gene inserted.

<p>The amplified product is marked with large arrow. L represents the DNA ladder; five bands are marked in kbp. ‘-ve’ represents the negative control that lacked the insert during overlapping PCR reaction. The final product is seen only in those reactions that had the gene of interest. Although other DNA bands are also seen on the gel, they were not identified and, possibly, did not interfere in our experiment.