<i>In vitro</i> characterization of IFN-I reporters.

<p>A) Schematic representation of reporter plasmid constructs. B) Dual luciferase reporter assay in HuH-7 cells transfected with the indicated plasmids in response to 500 units/ml of human IFN-α for 24 hours. C) Luciferase activity (fold induction) in HuH-7 cells transfected with the indicated reporter plasmids and treated with 500 units/ml of human IFN-α for 24 hours. D) Dual luciferase reporter assay in Hepa 1.6 cells transfected with the indicated plasmids in response to Sendai Cantell virus (20 hemagglutination units, 24 hours). E) Fold induction of luciferase activity in cells transfected with the 3xIRF-ISRE reporter plasmids in the indicated cell lines treated during 24 hours with 500 units/ml of the corresponding species-specific IFN-α. F) Luciferase activity fold induction in murine cell lines transfected with the 3xIRF-ISRE reporter plasmid and treated with 500 units/ml murine IFN-α, 500 units/ml murine IFN-β, 50 μg/ml poly I:C or 1 μg/ml poly I:C mixed with 250 μg/ml DEAE-Dextran for 24 hours. G) Time course of luciferase activity in HuH-7 cells transfected with the 3xIRF-ISRE-luc reporter plasmids and treated with 500 units/ml of human IFN-α. These data are from one experiment representative of four. *** p<0.001, ns: not significant. TK: Thymidine kinase.