IFN system analysis in PH5CH8 cell line.

<p><b>A)</b> Fold induction of IFN-β promoter-Luciferase reporter gene in HCC cell lines (HepG2 and Huh-7), immortalized hepatocytes (PH5CH8) and primary human hepatocytes (PHH). Cells were transfected with the reporter plasmid containing the firefly luciferase gene under the control of the IFN-β promoter. At 24 hours post-transfection, cultures were stimulated by a second round of transfection with Poly (I:C) (T-pIC), Poly (I:C) was added to the medium (M-pIC), or infected with VSV-wt or VSV-M51R. IFN-luciferase activities were measured and normalized to Renilla luciferase (RL) gene used as an internal control. Significance was calculated by comparison with mock-treated cultures expressing basal firefly luciferase activity (* p<0.05; ** p<0.01; ***p<0.001). <b>B)</b> Interferon protection assay in PH5CH8 compared to PHH and HepG2 and Huh-7 cells as representatives for HCC. Cells were treated overnight with 500 IU/ml of universal type I interferon (IFN) or simply mock-treated. VSV-wt infection was performed at MOI of 1 and viral titers were obtained 24 hr post-infection. Titers are the mean of at least three independent experiments (* p<0.05).