G6PDH activity of wild type and mutant enzymes as a function of NAD⁺ or NADP⁺ concentration.

<p>NADP⁺-dependent activity (black circles) and activity with NAD⁺ (white circles) was measured at nearly saturating co-substrate concentrations (bottom right corner). The data was fitted to the Michaelis-Menten equation and the Pearson correlation coefficient is indicated. The values for the kinetic parameters are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0152403#pone.0152403.t001" target="_blank">Table 1</a>.