Effect of the substrate concentration on the initial rate (nmol/min) of the hydrolysis reaction catalyzed by the wild-type and mutant bglTm proteins.

<p>(A) Wild-type bglTm. (B) Mutant T1. (C) Mutant T2. The tested substrates were <i>p</i>-nitrophenyl β-glucopyranoside (NPβGlc) and <i>p</i>-nitrophenyl β-fucopyranoside (NPβFuc). Lines indicate the best fits of the experimental data (dots) using the appropriate kinetic equation (see the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0191282#sec002" target="_blank">Materials and Methods</a> for details).