JEV NS2B(H)-NS3(pro) catalysed substrate hydrolysis rates at different substrate concentrations.

<p>The graph shows reaction velocities of JEV NS2B(H)-NS3(pro) for hydrolysis of Boc-GRR-amc (A); Boc-LRR-amc (B); Ac-nKRR-amc (C), Bz-nKRR-amc (D), Pyr-RTKR-amc (E), and Abz-(R)₄SAGnY-amide (F). Assays were performed at 37°C in 50 mM Tris-HCl, pH 9.5, 30% v/v glycerol. Kinetic parameters were determined by non-linear fitting of untransformed data to the Michaelis-Menten equation. Data are reported as the mean of three experiments ± standard error (SEM).