Relative transcriptional activities of wild type and variant SIRT3 gene promoters in H9c2 cells.

<p>Wild type and variant SIRT3 gene promoters were cloned into reporter gene vector pGL3 and transfected into H9c2 cells. Dual-luciferase activities were assayed and relative activities of SIRT3 gene promoters were obtained. Transcriptional activity of the wild type SIRT3 gene promoter was designed as 100%. Lanes 1, pGL3-WT; 2, pGL3 control; 3, pGL3-g.237312T>C (rs3817629); 4, pGL3-g.237307A>G; 5, pGL3-g.237270G>A + g.236370C>G + g.236380_81ins; 6, pGL3-g.237030C>T (rs12293349); 7, pGL3-g.237023_25del; 8, pGL3-g.237022C>G (rs369344513); 9, pGL3-g.236956G>T; 10, pGL3-g.236878_79Ins; 11, pGL3-g.236775del (rs369178836); 12, pGL3-g.236653C>A + g.236473C>T; 13, pGL3-g.236634C>T; 14, pGL3-g.236628G>C; 15, pGL3-g.236583A>C; 16, pGL3-g.236557T>C; 17, pGL3-g.236460T>C. *, P<0.05; **, P<0.01.